Fig. 3.

Impaired activation of caspases and JNK in Irf5^−/− livers. (A) Liver extracts were prepared from WT and Irf5^−/− mice 3 h after i.p. injection of Jo2 mAb [indicated by (+); three mice were analyzed] or PBS [indicated by (−)]. Whole-cell extract from thymocytes were prepared 18 h after the addition of Jo2 mAb and protein A. Immunoblot analysis was performed by using specific antibodies as indicated. β-Actin was used as a loading control. (B) Immunoblot analysis for liver extracts was performed as in A using the indicated antibodies. Liver extracts from two mice injected with Jo2 mAb were analyzed.