Fig. 4.
qRT-PCR analysis of WT and Irf5−/− livers. (A) qRT-PCR for Irf5 was performed by using RNA purified from WT mice 3 h after injection of Jo2 mAb or PBS. For comparison, RNA from WT MEFs before and 12 h after vesicular stomatitis virus (VSV) treatment also was analyzed. (B) qRT-PCR for the indicated transcripts was performed by using RNA purified from WT and Irf5−/− livers as in A. The Cflar primers detect the transcripts for both cFLIPL and cFLIPS, and the primers that specifically detect cFLIPL also gave a similar result (data not shown). The primers for Bim detect a common region for BimEL, BimL, and BimS.