Fig. 2.

Ipaf responds to P. aeruginosa that expresses both T3SS and flagellin. (A) Schematic of T3SS mutants. (B) WT BMMs were stimulated with LPS to promote proIL-1β expression and infected with WT or mutant P. aeruginosa. IL-1β expression was determined in response to T3SS mutants lacking the Psc apparatus (pscC) or translocon (popB or popD). (C) Caspase 1 processing was determined by Western blot from macrophages infected with WT or pscC mutant P. aeruginosa. (D) IL-12 p40 secretion was determined by ELISA from macrophages infected with P. aeruginosa strains for 1 h, washed, and treated with gentamicin for four additional hours. The asterisk denotes a nonspecific band.