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. 2008 Feb 6;105(7):2562–2567. doi: 10.1073/pnas.0712183105

Fig. 3.

Fig. 3.

Ipaf responds to P. aeruginosa expressing flagellin. (A) Diagram of flagellar structure formed by WT P. aeruginosa or the amotile flgK, fliC, and flgB mutants. (B) IL-1β secretion by LPS-primed WT macrophages infected with WT P. aeruginosa, amotile mutants expressing flagellin monomer (flgK), or amotile mutants not expressing flagellin monomer (fliC or flgB). (C) Caspase 1 processing in macrophages infected with P. aeruginosa mutants was examined by Western blot. (D) IL-12 p40 secretion was determined by ELISA from macrophages infected with P. aeruginosa strains for 1 h, washed, and treated with gentamicin for four additional hours. (E) The motility phenotype of strains was confirmed in soft agar. The asterisk denotes a nonspecific band.