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Ipaf responds to cytosolic P. aeruginosa flagellin. WT, TLR5-null, or Ipaf-null BMMs were stimulated with LPS to promote pro-IL-1β expression before stimulation with P. aeruginosa flagellin with or without protein transfection reagent (Profect P1). IL-1β secretion was determined by ELISA 1 h later.
