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. 2008 Feb 7;105(7):2568–2573. doi: 10.1073/pnas.0711593105

Fig. 3.

Fig. 3.

Knockdown of WIP prevents granule but not F-actin polarization to the immune synapse in NK cells. YTS (A) and pNK (B) cells, untransfected (Top) or transfected with empty RNAi vector (Middle) or WIP RNAi (Bottom), were activated by mixing with 721.221 target cells for 15 min at 37°C. Cells were next stained with AlexaFluor 568-conjugated phalloidin (red) and AlexaFluor 647-conjugated anti-perforin antibodies (green) to visualize F-actin and lytic granules, respectively. (Scale bar: 5 μm.) (C) The percentages of F-actin and perforin polarization toward the cell–cell contact site (immune synapse) in YTS (Left) and pNK (Right) cells conjugated with 721.221 target cells. Error bars represent SD. The values were determined by evaluation of 100–200 conjugates in two to four separate experiments. The asterisks indicate statistical significance (*, P < 0.05; **, P < 0.01 by Student's t test) when compared with control.