Fig. 1.

Properties of HIV gag-specific CD4⁺ T cells after prime-boost immunization with α-DEC-p24 and poly IC. C57BL/6 mice were injected s.c. with 5 μg of α-DEC-p24 and 2, 10, or 50 μg of poly IC or PBS, and boosted with the same conditions 6 wk later. One other group received 5 μg of α-DEC-p24 and 50 μg of poly IC once at the time of the boost. Two weeks later, HIV gag-specific, CD3⁺ CD4⁺, splenic T cells were analyzed for IFN-γ-, IL-2-, and TNF-α-secretion (A), percentage of CD4⁺ T cells expressing each of seven possible combinations of IFN-γ, TNF-α, and IL-2 (B), and IFN-γ, TNF-α, and IL-2 MFIs of gag-specific cells expressing 3⁺, 2⁺, or 1⁺ cytokines after prime-boost with α-DEC-p24 plus 50 μg of poly IC (C). Data are representative of two experiments with two mice pooled in each.