Fig. 5.

Poly IC₁₂U acts as an adjuvant for CD4⁺ T cell immunity to α-DEC-p24 vaccine in a TLR3-dependent manner. (A) CxB6 F₁ mice were injected i.p. with 5 μg of α-DEC-p24 plus graded doses of poly IC (InVivoGen) or poly IC₁₂U (Ampligen) or PBS, and boosted with the same conditions 6 wk later. The percentage of IFN-γ-producing and proliferating CD3⁺CD4⁺ T cells in response to HIV gag p17 or p24 mix 1 wk after boost are shown as mean ± SD (n = 4 mice). (B) IFN-γ-secretion in response to HIV gag p24 peptides by CD4⁺ splenocytes in wild-type, TLR3^−/−, and MDA5^−/− mice immunized with two doses of α-DEC-p24 plus 50 μg of poly IC (InVivoGen) or 250 μg of poly IC₁₂U (Ampligen). Data are one of two similar experiments with two mice in each.