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. 2008 Feb 8;105(7):2699–2704. doi: 10.1073/pnas.0711278105

Fig. 3.

Fig. 3.

GPR55 activation releases calcium from IP3R-mediated calcium stores via phospholipase C activation. (A) A subset of hGPR55-HEK293 cells was perfused with either THC or JWH015 in 0 Ca2+ Ringer's solution, and the changes in [Ca2+]i were compared with those in cells perfused with THC or JWH015 in control Ringer's solution (2 mM Ca2+). (B) Summary calcium responses in hGPR55-HEK293 cells pretreated with 1 μM thapsigargin (TG), or coperfused with either THC or JWH015, in combination with either the IP3R inhibitor xestospongin D (XeD, 1 μM) or the RyR inhibitor dantrolene (Dan, 10 μM). These calcium rises were compared with those seen in control cells. (C) Summary calcium responses in large DRG neurons pretreated with 1 μM thapsigargin (TG) and perfused with THC. (D) Calcium changes in a subset of hGPR55-HEK293 cells treated with the PI-specific PLC inhibitor, ET-18-OCH3 (edelfosine), relative to controls, upon perfusion of THC or JWH015.