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. 2008 Feb 12;105(7):2717–2722. doi: 10.1073/pnas.0712300105

Fig. 2.

Fig. 2.

Caspase activity after exposure to GAL was measured with the fluorimetric DEVDase assay. Exposure of transfected GalR2 cells to 100 nM GAL induced a time-dependent significant increase in DEVDase activity (A). Exposure to different concentrations of GAL for 24 h caused a dose-dependent increase (B). A significant DEVDase activity was also induced after 24 h exposure to AR-MI896 only in transfected cells (C). Transfected cells were preincubated with the pan-caspase inhibitor z-VAD-fmk for 30 min before exposure to GAL for 48 h and then fixed with methanol and stained with Hoechst dye 33358. z-VAD-fmk significantly decreased the number of apoptotic nuclei (D). Values are means ± SEM of three determinations. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.