Skip to main content
. 2008 Feb 4;105(7):2723–2728. doi: 10.1073/pnas.0712116105

Fig. 5.

Fig. 5.

Molecular mechanism of hypernociception. (A) Mice were pretreated with IL-1ra, bosentan, clazosentan, or indomethacin as described in Fig. 1 and then injected i.pl. with 100 pg of TNFα. Hypernociception was determined 3 h after TNFα injection. (B) TNFα failed to induce hypernociception in IFNγ−/− mice. (C) Mice were pretreated with bosentan, clazosentan, or indomethacin before injected i.pl. with 1 ng of IL-1β, and hypernociception was determined as above. (D and E) IL-1β induced normal hypernociception in TNFR1−/− mice (D), but failed to do so in IFNγ−/− mice (E). (F) IFNγ (3 ng)-induced hypernociception was blocked by bosentan and indomethacin, but not by IL-1ra. Data are means ± SEM (n = 5), representative of two independent experiments. *, P < 0.05, compared with saline group; #, P < 0.05, compared with the vehicle/WT groups.