TABLE 1.
Oligonucleotide primers used in this studya
Primer | Purpose | Localization | Tm (°C) | Nucleotide sequence (5′→3′) | Reference or source |
---|---|---|---|---|---|
UMS-125 | MBA amplification | MBA 5′ | 66 | GTATTTGCAATCTTTATATGTTTTCG | 43 |
UMA1586 | MBA amplification | MBA 3′ | 72 | GATAATCATTCATCTTCTCTTAATTGTC | 43 |
UMSP88 | MBA amplification, control positive clones, sequencing | MBA 5′ | 55 | TGTTCTAATTCAACTGTTAAATCT | Present study |
UMAUA | MBA amplification, control positive clones, sequencing | MBA 3′ | 62 | GGGKWGTTKHACCAYTKCCTGGTT | 19 |
pTrcHis Forward | Control positive clones, sequencing | Cloning vector | 53 | GAGGTATATATTAATGTATCG | Invitrogen |
pTrcHis Reverse | Control positive clones, sequencing | Cloning vector | 48 | GATTTAATCTGTATCAGG | Invitrogen |
Oligonucleotide primers were synthesized by Eurogentec (Luik, Belgium).