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. 2007 Dec 26;15(3):549–561. doi: 10.1128/CVI.00351-07

FIG. 2.

FIG. 2.

Cross-reactions of MAbs generated against the DENV-2 NS1 glycoprotein with the DENV-2 E glycoprotein. Two concentrations (1,163 ng [lanes 2, 4, 6, 8, 10, 12, 14, and 16] and 292 ng [lanes 3, 5, 7, 9, 11, 13, 15, and 17]) of purified DENV-2 particles were subjected to SDS-PAGE and electroblotted onto nitrocellulose membranes. Purified MAbs 1H7.4 (lanes 2 and 3), 5H4.3 (lanes 4 and 5), 3D1.4 (lanes 6 and 7), 1G5.3 (lanes 8 and 9), 1G5.4-A1-C3 (lanes 10 and 11), and 1C6.3 (lanes 12 and 13), generated against the DENV-2 NS1 glycoprotein, MAb 2C5.1 (lanes 14 and 15), generated against the DENV-2 E glycoprotein, and pooled mouse PAbs generated in response to repeated live DENV-2 infections (lanes 16 and 17) were diluted 1/200 (MAb 1C6.3 and PAbs) or 1/400 (all other MAbs), incubated with each membrane strip, and detected using an enzyme-labeled secondary antibody and substrate. The locations of each molecular size (kDa) marker and of the DENV-2 E (gp60), gp55 (truncated pg60), iNS1 (gp46), and prM (gp20) glycoproteins are shown.