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. 2007 Dec 26;15(3):549–561. doi: 10.1128/CVI.00351-07

TABLE 2.

Reactions of MAbs generated to the DENV-2 NS1 glycoprotein with sequential epitopes represented by synthetic peptides from the DENV-2 NS1 glycoprotein

Peptide sequencea Epitopeb Average MAb ELISA absorbance (% of maximum absorbance)c
1H7.4 5H4.3 3D1.4 1G5.3 1G5.4-A1-C3 1C6.3
5-VVSWKNKELKC-15 N terminus 0.03 0.04 0.11 0.15 0.66 (57.8) 0.71 (58.2)
25-VHTWTEQYK-33 LD2 2.14 0.21 0.09 0.25 0.80 (70.2) 0.76 (62.3)
61-TRLENLMWK-69 24A 0.04 2.26 0.08 0.22 0.86 (75.4) 0.66
109-TELKYSWKT-117 N'LX1 0.05 0.05 0.22 0.05 1.14 (100.0) 0.55
113-YSWKTWGKA-121 LX1 0.06 0.02 2.02 (76.8) 0.03 0.33 0.15
113-YSWKTWG-119 LX1 core 0.03 0.02 2.63 (100.0) 0.03 0.21 0.09
208-TWKIEKASF-216 LX2/1 0.03 0.02 0.06 0.28 0.45 1.22 (100.0)
267-PWHLGKLEM-275 LX2/2 0.03 0.02 0.07 0.30 0.52 1.02 (83.6)
301-TTASGKLIT-309 24C 0.03 0.03 0.10 3.88 (100.0) 0.32 0.18
302-TASGKL-307 24C core 0.03 0.02 0.05 3.01 (77.6) 0.11 0.06
331-YGMEIRPLK-339 LX2/3 0.05 0.05 0.07 0.06 0.85 (74.6) 0.88 (72.1)
a

Single-letter amino acid code for synthetic peptide sequences from the DENV-2 NS1 glycoprotein. The ELK/KLE-type and KELK/KLEK-type motifs are underlined.

b

Epitope on the DENV-2 NS1 glycoprotein with the full or core epitope sequences.

c

Average ELISA reactions of MAbs generated to the DENV-2 NS1 glycoprotein, diluted to 100 times their ELISA titer against the DENV-2 NS1 glycoprotein, and incubated with duplicate sets of each peptide sequence (standard deviation range of absorbance values, 0.00 to 0.02). Values above the absorbance cutoffs of 0.650 and 1.000 are shown in bold and underlined, respectively, and the percentages of maximum absorbance obtained against the 25-33, 61-69, 113-119, 301-309, 109-117, and 208-216 peptide sequences, using MAbs 1H7.4 (314 ng/ml), 5H4.3 (1,714 ng/ml), 3D1.4 (442 ng/ml), 1G5.3 (575 ng/ml), 1G5.4-A1-C3 (1,595 ng/ml), and 1C6.3 (4,439 ng/ml), respectively, are shown in parentheses.