Western blot analysis of crude extracts from T. spiralis adult worms (lane 1), T. spiralis newborn larvae (lane 2), T. spiralis 15-day p.i. muscle larvae (lane 3), T. spiralis 30-day p.i. muscle larvae (lane 4), E-S products from T. spiralis muscle larvae (lane 5), crude extracts from T. pseudospiralis adult worms (lane 6), T. pseudospiralis newborn larvae (lane 7), T. pseudospiralis 15-day p.i. muscle larvae (lane 8), T. pseudospiralis 30-day p.i. muscle larvae (lane 9), and E-S products from T. pseudospiralis muscle larvae (lane 10) immunostained with antibody against the T. spiralis 53-kDa recombinant protein (A) or immunostained with antibody against the T. pseudospiralis 53-kDa recombinant protein (B). M, molecular mass standards; sizes in kDa are shown on the left side.