FIG. 1.

Immunogenicity of S-3 versus that of P-3. (A) Frequency of Th1/Th2 lymphocytes among ex vivo-stimulated splenocytes from BALB/c mice (eight mice per group) vaccinated with S-A, S-3, P-A, or P-3. Three weeks after the vaccination, the splenocytes were harvested and stimulated for 4 days with rAls3p-N or tetanus toxoid (TT). Medians and interquartile ranges are shown. *, P was ≤0.05 versus all other groups. (B) Culture supernatant cytokines induced by rAls3p-N stimulation of the splenocytes from the vaccinated or control mice. **, P was ≤0.03 versus all other groups; *, P was ≤0.05 versus S-A or P-A controls; †, P was 0.08 versus S-A.