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Reactivities of sera from leptospirosis patients with protein antigens. Sera were obtained from patients 2 days (lane 1), 16 days (lane 2), 6 days (lane 4), and 11 days (lane 5) after the onset of leptospirosis. Lane 3 shows the locations of molecular size standards. Lane 6 is an immunoblot of leptospiral antigens probed with antisera to leptospiral proteins GroEL, LipL41, LipL32, and LipL21.
