TABLE 3.
Distribution of IgM reactive immunoblot bands in 188 serum samples collected from 74 patients with laboratory-confirmed cases of leptospirosis, compared with conventional MAT and culture resultsb
| Antigen band(s) (kDa) | % of serum samples with IgM reactivity in days after fever onset
|
||||||
|---|---|---|---|---|---|---|---|
| 1 to 3 (n = 51) | 4 to 6 (n = 41) | 7 to 9 (n = 22) | 10 to 12 (n = 8) | 13 to 15 (n = 10) | 16 to 18 (n = 45) | 19 to 28 (n = 11) | |
| 41/42 | 27.5 | 31.7 | 31.8 | 50.0 | 50.0 | 26.7 | 54.5 |
| 37 | 21.6 | 9.8 | 36.4 | 25.0 | 30.0 | 17.8 | 27.3 |
| 35/36 | 23.5 | 22.0 | 31.8 | 12.5 | 40.0 | 26.7 | 36.4 |
| 32 | 47.1 | 51.2 | 59.1 | 87.5 | 80.0 | 75.6 | 63.6 |
| 24-30a | 31.4 | 39.0 | 54.5 | 75.0 | 30.0 | 66.7 | 63.6 |
| 19-23a | 76.5 | 73.2 | 81.8 | 100.0 | 80.0 | 77.8 | 100.0 |
| 14-18a | 82.4 | 85.4 | 81.8 | 100.0 | 80.0 | 82.2 | 100.0 |
| 14-18 and/or at least two in 19-42 region | 88.2 | 90.2 | 90.9 | 100.0 | 90.0 | 84.4 | 100.0 |
Diffuse banding was determined by a single serum sample, with immunoreactive bands commonly detected in the range of 14 to 18, 19 to 23, and 24 to 30 kDa.
For MAT, a single serum with a titer of ≥400 was considered positive. With MAT, the percentages of serum samples with IgM reactivity 1 to 3, 4 to 6, 7 to 9, 10 to 12, 13 to 15, 16 to 18, and 19 to 28 days after the onset of fever were 2.0, 22.0, 68.2, 87.5, 30.0, 75.6, and 63.6%, respectively. For culture, the percentages of samples with leptospire positivity at 1 to 3, 4 to 6, 7 to 9, 10 to 12, and 13 to 15 days after the onset of fever were 45.9% (34/74 samples), 10.8% (8/74 samples), 2.7% (2/74 samples), 1.4% (1/74 samples), and 1.4% (1/74 samples), respectively.