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Induction of anti-PspA antibodies in sera from BALB/c WT and IL-4 KO mice immunized with recombinant protein or DNA vaccine. Total anti-PspA IgG (A) or anti-PspA IgG1 and IgG2a (B) antibodies were detected through ELISA in plates coated with rPspA3NS, using sera obtained from individual mice (six animals per group) 2 weeks after the last immunization. Numbers above columns are mean IgG1/IgG2a titer ratios.
