FIG. 4.

Selected examples of motifs in the discriminator region for cofactor binding. N-terminal TyrA sequence patterns that distinguish specificity for NAD⁺ (top), specificity for NADP⁺ (middle), and the ability to accept either cofactor [NAD(P)⁺] (bottom) are shown. Sequences shown begin with the last G (residue 11) of the GxGxxG motif in the Wierenga fingerprint (73). The variable gap of the Wierenga fingerprint is shown as a gray column. Examples of the smallest gap (one residue) and the largest gap (nine residues) are given. Two different patterns are shown for the NADP⁺ category, and two patterns are shown for the broad-specificity category. Motifs that center around the all-important residue 36 are shown for each of the five groups.