TABLE 1.
Plasmid, phage, and bacterial strains used in this study
| Bacterial strain, phage, or plasmid | Description | Source or reference(s) |
|---|---|---|
| Bacterial strains | ||
| E. coli Top10 | Commercial strain used as a cloning host | Invitrogen |
| E. coli HB101 | E. coli strain without F factor | 9 |
| M. smegmatis mc2 155 | High-frequency transformation derivative of M. smegmatis mc2 6 | 36 |
| M. avium subsp. paratuberculosis K10 | Virulent clinical isolate and sequencing project strain | 13, 18 |
| M. avium subsp. paratuberculosis K10-GFP | M. avium subsp. paratuberculosis K10 containing pWES4 for GFP expression | 22 |
| Phage or plasmid | ||
| phAE87 | Conditionally replicating shuttle phasmid derivative of TM4 | 4 |
| pYUB 854 | Derivative of pYUB572; bla gene was replaced with hyg cassette | 5 |