TABLE 4.
Effect of recovery time on the efficiency of allelic exchange mutagenesis
| Recovery time (h) | ΔrelAL K10
|
ΔrelAL K10-GFP
|
Δlsr2K10
|
|||
|---|---|---|---|---|---|---|
| No. of ΔrelAL mutants/no. of Hygr mutants (%)a | Total no. of Hygr mutants | No. of ΔrelAL mutants/no. of Hygr mutants (%)a | Total no. of Hygr mutants | No. of Δlsr2 mutants/no. of Hygr mutants (%)a | Total no. of Hygr mutants | |
| 0 | 50/50 (100) | 656 | 39/50 (78) | 266 | 50/50 (100) | 402 |
| 24 | 48/50 (96) | 291 | 42/50 (84) | 448 | 50/50 (100) | 738 |
a
Values in parentheses are the allelic exchange frequencies.