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. 2008 Jan 25;74(6):1767–1779. doi: 10.1128/AEM.01668-07

FIG. 2.

FIG. 2.

Effects of various concentrations of PI (A and B) and SG1 (C and D) on the enumeration of “live” (○) and “dead” (•) cells in a sample of Mediterranean seawater subjected at 70°C in a water bath for 1 min (A and C) and the same seawater sample subjected for 5 min at 70°C (B and D). Panels A and B show results for staining with various concentrations of PI and a fixed SG concentration of 10×. Panels C and D show results for staining with various concentrations of SG and a fixed concentration of 10 μg ml−1 of PI. (E) Evolution of the percentages of live cells at different concentrations of PI with a fixed SG concentration (10×). (F) Evolution of the percentages of live cells at different concentrations of SG with a fixed PI concentration (10 μg ml−1). Symbols: ▪, a sample maintained at 70° for 1 min; □, a sample maintained at 70° for 5 min. Arrows in panels A to D indicate the determined optimal concentrations of PI and SG.