TABLE 1.
Characteristics of the different versions of the NADS protocol found in the literaturea
| Type of sample | Stain 1 | Concn (×) | Dilution (vol/vol) | Stain 2 | Concn (μg ml−1) | Note | Reference |
|---|---|---|---|---|---|---|---|
| Freshwater | SG1 | 0.1 | 1:100,000 | PI | 1 | For fewer than 0.5 × 106 bacteria ml−1 | 29 |
| Freshwater | SG1 | 1 | 1:10,000 | PI | 10 | For up to 20 × 106 bacteria ml−1 | 29 |
| Seawater | SG2 | 10 | 1:1,000 | PI | 10 | 29 | |
| Seawater | SG2 | 10 | 1:1,000 | PI | 10 | 30 | |
| Seawater | SG1 | 1 | 1:10,000 | PI | 1-40 | Range of PI studied | 44 |
| Drinking water | SG2 | 5 | 1:2,000 | PI | 10 | 56 | |
| Seawater | SG1 | 25 | 1:400 | PI | 0.6 | 58 | |
| Seawater | SG1 | 1 | 1:10,000 | PI | 10 | 1 | |
| Freshwater | SG | 10 | 1:1,000 | PI | 2 | Does not specify which SG | 9 |
a
SG2, Sybr green II.