Table 2.
Leukocyte rolling velocities and wall shear rates in mouse cremaster venules
| Early (<30 min after exteriorization) | ||||
|---|---|---|---|---|
| Wild type | L-selectin−/− | ICAM-1−/− | L-selectin/ICAM-1−/− | |
| Rolling vel. (μm/s) | 36 ± 0.2 (65) | 19 ± 0.1 (105)* | 47 ± 0.3 (95) | 40 ± 0.2 (120) |
| Wall shear rate (s−1) | 608 ± 19 (13) | 575 ± 8 (19) | 551 ± 8 (21) | 654 ± 8 (24) |
| Late (>60 min after exteriorization) | ||||
| Rolling vel. (μm/s) | 38 ± 0.1 (140) | 24 ± 0.1 (365)* | 58 ± 0.4 (100)‡ | 64 ± 0.1 (340)†§ |
| Wall shear rate (s−1) | 560 ± 2 (73) | 580 ± 8 (28) | 564 ± 11 (20) | 559 ± 3 (68) |
| TNF-α (3 hr) | ||||
| Rolling vel. (μm/s) | 4.7 ± 0.6 (247) | 6.4 ± 0.5 (164) | 8.8 ± 0.8 (105)‡¶ | 8.2 ± 0.6 (150)‡ |
| Wall shear rate (s−1) | 526 ± 4 (56) | 529 ± 65 (33) | 461 ± 42 (14) | 491 ± 36 (28) |
Mean ± SEM of (n) vessels or cells, respectively. vel. = velocity. The TNF-α data from wild-type and L-selectin−/− mice were published previously (12).
*
Significantly different from all other genotypes at the same time pont (P < 0.01).
†
Significantly different from the same genotype at the <30 min time pont (P < 0.01).
§
Significantly different from wild-type at the same time point (P < 0.01),
‡
(P < 0.05).
¶
Significantly different from L-selectin−/− mice (P < 0.05).