TABLE 1.
Primers used for PCRs in this study to detect and identify ciliated protozoa in pure cultures and stream biofilm samples
| Target and primer | Sequence (5′→3′) | Reference(s) or source |
|---|---|---|
| Eukaryotic 18S rRNA genes | ||
| 82FEa | GAA DTC GYG AAY GGC TC | 13, 26, 47 |
| EK-1520Rb | CYG CAG GTT CAC CTA C | 26, 47 |
| E528F | CGG TAA TTC CAG CTC C | 50 |
| 1391REc | GGG CGG TGT GTA CAA RGR G | 13, 50 |
| Ciliophora 18S rRNA genese | ||
| 121F | CTG CGA ATG GCT CAT TAM AA | This study |
| 384F | YTB GAT GGT AGT GTA TTG GA | This study |
| 1147R | GAC GGT ATC TRA TCG TCT TT | This study |
| 1147Fd | GAA CGA AAG WTA RGG GAT CA | This study |
| 2755Rd | CGT TSA WGA TCY ANA ATT NCA AAG | This study |
| 2824Rd | CAG GGA CKT ART CAR TGC AA | This study |
a
Referred to as 18S-82F, with sequence GAA ACT GCG AAY GGC TC, in reference 47 and EK-82F, with sequence GAA ACT GCG AAT GGC TC, in reference 26.
b
Referred to as 18S-1520R in reference 47.
c
Referred to as Univ1391RE in reference 50.
d
Designed and tested but excluded from further application in this study.
e
Primers were newly designed.