TABLE 3.
Summary of identity of 18S rRNA gene clones derived from biofilm DNA extracts from four streams using ciliate-specific primer combination 384F/1147Ra
| Sequence source (class or phylumb) | Cascade Stream (undeveloped native forest)
|
Stoney Creek (developed native forest)
|
Opanuku Stream (rural)
|
Pakuranga Stream (urban)
|
Total
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| No. of clones | No. of sequences | No. of clones | No. of sequences | No. of clones | No. of sequences | No. of clones | No. of sequences | No. of clones | No. of sequences | |
| Ciliates | ||||||||||
| Colpodea | 2 | 1 | 10 | 3 | 12 | 4 | ||||
| Litostomatea | 2 | 1 | 1 | 1 | 4 | 4 | 7 | 6 | ||
| Nassophorea | 4 | 2 | 4 | 2 | ||||||
| Oligohymenophorea | 20 | 6 | 14 | 5 | 26 | 8 | 41 | 6 | 101 | 23 |
| Phyllopharyngea | 14 | 4 | 10 | 2 | 24 | 6 | ||||
| Prostomatea | 2 | 1 | 2 | 1 | 1 | 1 | 5 | 3 | ||
| Spirotrichea | 5 | 2 | 4 | 2 | 11 | 6 | 2 | 2 | 22 | 9 |
| Uncultured ciliate | 1 | 1 | 1 | 1 | ||||||
| Total ciliates | 31 (60) | 11 (73) | 49 (70) | 18 (90) | 38 (86) | 15 (79) | 58 (78) | 15 (83) | 176 (73) | 54 (87) |
| Nonciliates | ||||||||||
| Amoebozoa | 9 | 1 | 2 | 1 | 1 | 1 | 12 | 1 | ||
| Apicomplexa | 9 | 1 | 9 | 1 | ||||||
| Basidiomycota | 14 | 1 | 14 | 1 | ||||||
| Cercozoa | 2 | 1 | 2 | 1 | ||||||
| Chlorophyta | 1 | 1 | 1 | 1 | ||||||
| Chytridiomycota | 3 | 2 | 19 | 1 | 3 | 2 | 1 | 1 | 26 | 3 |
| Total nonciliates | 21 | 4 | 21 | 2 | 6 | 4 | 16 | 3 | 64 | 8 |
a
Clones were identified according to RFLP profiles (data not shown) and BLAST matches. Values in parentheses are percentages of ciliates.
b
Class for ciliates and phylum for nonciliates.