FIG. 3.

Genotypes and phenotypes of S. erythraea HL3168 E3 and its recombinant strains. (A) Restriction maps showing predicted fragment sizes upon SmaI and/or SphI digestion. (B) Southern analyses of genomic DNAs digested with SmaI or SphI by using the 1.7-kb eryK-containing fragment as a probe. (C) HPLC analyses of the Er production in fermentations. I, HL3168 E3; II, ZL1001; III, ZL1002; IV, ZL1003; V, ZL1004; VI, ZL1005; VII, ZL1006; VIII, ZL1007; IX, ZL1008; X, ZL1009. Solid circles, stars, and triangles indicate Er-C, Er-A, and Er-B, respectively. Each wavy line indicates the DNA fragment of the vector integrated by single-crossover homologous recombination.