TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Host for general cloning | Invitrogen |
| ET12567(pUZ8002) | Donor strain for conjugation between E. coli and Streptomyces | 12 |
| S. erythraea | ||
| HL3168 E3 | Industrial Er-producing strain | 30 |
| ZL1001 | eryK-duplicated derivative of HL3168 E3, with the genotype PermK*-K + PermE*-K + PermA*-G | This work |
| ZL1002 | eryK and eryG-duplicated derivative of HL3168 E3, with the genotype PermK*-K-G + PermE*-K + PermA*-G | This work |
| ZL1003 | eryK and eryG-duplicated derivative of HL3168 E3, with the genotype PermK*-K + PermA*-G + PermE*-K-G | This work |
| ZL1004 | eryK-triplicated and eryG-duplicated derivative of HL3168 E3, with the genotype PermK*-K-K-G + PermE*-K + PermA*-G | This work |
| ZL1005 | eryK-triplicated and eryG-duplicated derivative of HL3168 E3, with the genotype PermK*-K-G + PermE*-K-K + PermA*-G | This work |
| ZL1006 | eryK-triplicated and eryG-duplicated derivative of HL3168 E3, with the genotype PermK*-K + PermA*-G + PermE*-K-K-G | This work |
| ZL1007 | eryK-triplicated and eryG-duplicated derivative of HL3168 E3, with the genotype PermK*-K-G-K + PermE*-K + PermA*-G | This work |
| ZL1008 | eryK-triplicated and eryG-duplicated derivative of HL3168 E3, with the genotype PermK*-K + PermE*-K-G-K + PermA*-G | This work |
| ZL1009 | eryK-triplicated and eryG-duplicated derivative of HL3168 E3, with the genotype PermK*-K + PermA*-G-K + PermE*-K-G | This work |
| Plasmids | ||
| pGEM-T Easy | E. coli subcloning vector | Promega |
| pGEM-7zf | E. coli subcloning vector | Promega |
| pANT841 | E. coli subcloning vector | 32 |
| pWMH3 | E. coli-Streptomyces shuttle vector; Thior | 29 |
| pKC1139 | E. coli-Streptomyces shuttle vector; Aprr; temperature-sensitive replication origin carrier | 3 |
| pZL1001 | 1.7-kb PCR product that contains eryK and its downstream native terminator in pGEM-T Easy | This work |
| pZL1002 | 2.2-kb fragment that contains the PermE*-controlled eryK and its downstream native terminator in pWHM3 | This work |
| pZL1003 | 2.2-kb fragment that contains the PermE*-controlled eryK and its downstream native terminator in pKC1139 | This work |
| pZL1004 | 1.3-kb PCR product that contains eryK without its downstream native terminator in pANT841 | This work |
| pZL1005 | 1.8-kb fragment that contains the PermE*-controlled eryK in pANT841 | This work |
| pZL1006 | 1.5-kb PCR product that contains eryG in pANT841 | This work |
| pZL1007 | 1.5-kb fragment that contains eryG in pET-28a | This work |
| pZL1008 | 3.3-kb fragment that contains the PermE*-controlled eryK-G in pANT841 | This work |
| pZL1009 | 3.3-kb fragment that contains the PermE*-controlled eryK-G in pKC1139 | This work |
| pZL1010 | 1.3-kb PCR product that contains eryK without its downstream native terminator in pGEM-7zf | This work |
| pZL1011 | 4.6-kb fragment that contains the PermE*-controlled eryK-K-G in pANT841 | This work |
| pZL1012 | 4.6-kb fragment that contains the PermE*-controlled eryK-G-K in pANT841 | This work |
| pZL1013 | 4.6-kb fragment that contains the PermE*-controlled eryK-K-G in pKC1139 | This work |
| pZL1014 | 4.6-kb fragment that contains the PermE*-controlled eryK-G-K in pKC1139 | This work |