TABLE 3.
Designations, sources, similarities, and functions of the new enzymes from the GH13 family
| Enzyme | Source | Location (conditions) | Closest match for:
|
Closest structure-determined homolog by similarity
|
Main activity (exptl, this work) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Conserved fragment, regions I and II
|
Complete sequence
|
||||||||||||
| Enzyme | Organism | Enzyme | Organism | Swiss-Prot accession no. | Identity (%) | Enzyme | Organism | Swiss-Prot accession no. | Identity (%) | ||||
| Amy1 | Environmental DNA (oligotrophic enrichment) | Hveragerdi (60-65°C, pH 7-8) | CDase | Alicyclobacillus acidocaldarius | CDase | Alicyclobacillus acidocaldarius | Q9WX32 | 80 | Maltogenic amylase | Thermus sp. | O69007 | 42 | CDase |
| Neopullulanase 2/ TVAII | T. vulgaris | Q08751 | 41 | ||||||||||
| Amy29 | Environmental DNA (oligotrophic enrichment) | Hveragerdi (60-65°C, pH 7-8) | Neopullulanase | Synechocystis sp. | Neopullulanasea | T. thermophilus | Q5SI17 | 64 | Maltogenic amylaseb | Thermus sp. | O69007 | 44 | Neopullulanase |
| Amy132 | Environmental DNA (oligotrophic enrichment) | Grensdalur (65°C, pH 6-7) | Neopullulanase | G. stearothermophilus | CDase | Anoxybacillus flavithermus | Q5BLZ6 | 76 | Neopullulanase | G. stearothermophilus | P38940 | 67 | CDase |
a
Domain composition similar to that for query ABA′C.
b
Domain composition: NABA′C.