TABLE 5.
Molecular, catalytic, and thermophilic properties of Amy29
| Parameter | Result for Amy29 |
|---|---|
| Apparent molecular mass (kDa) | |
| Native | 90 |
| Subunit | 45 |
| Oligomeric structure | α2 |
| Preferred substrate | Pullulan |
| Vmax (65°C) (U mg−1) | 25 |
| Km (65°C) (g liter−1) | 4.5 |
| Product | Panose |
| pH optimum | 5.3 |
| Temp optimum (°C) | 65 |
| Thermostability | |
| Half-life at 65°C (min) | No loss of activity over 120 min |
| Half-life at 80°C (min) | >480 |
| Cation dependence (0.01 to 50 mM) | |
| Mn2+ | Threefold |
| Ca2+ | No effect |
| Cu2+ (below 0.1 mM) | Complete inhibition |
| Effect of EDTA treatment | None |