TABLE 1.
Bacterial strains and vectors used for this study
| Strain or vector | Description | Source or reference |
|---|---|---|
| Strains | ||
| EC1000 | E. coli strain containing a chromosomal copy of the pWV01 repA gene; Kanr | 15 |
| ATCC 55730 | L. reuteri strain isolated from human breast milk | Biogaia, AB, Sweden |
| PRB190 | L. reuteri ATCC 55730 clpL (lr1864) mutant; Emr | 42 |
| PRB188 | L. reuteri ATCC 55730 putative esterase (lr1516) mutant; Emr | 42 |
| PRB167 | lr0085::pKW01 in an ATCC 55730 background; Emr | This study |
| PRB186 | L. reuteri ATCC 55730 clpE (lr0004) mutant; Emr | Stefan Roos |
| PRB126 | lr1265::pKW02 in an ATCC 55730 background; Emr | This study |
| PRB130 | lr1584::pKW03 in an ATCC 55730 background; Emr | This study |
| PRB163 | lr1291::pKW04 in an ATCC 55730 background; Emr | This study |
| PRB125 | lr1351::pKW05 in an ATCC 55730 background; Emr | This study |
| PRB114 | lr1706::pKW06 in an ATCC 55730 background; Emr | This study |
| Vectors | ||
| pVE6007 | CmrrepA-positive temperature-sensitive derivative of pWV01 | 19 |
| pORI28 | EmrrepA-negative derivative of pWV01 | 15 |
| pKW01 | pORI28 + 203-bp insert from lr0085 | This study |
| pKW02 | pORI28 + 303-bp insert from lr1265 | This study |
| pKW03 | pORI28 + 331-bp insert from lr1584 | This study |
| pKW04 | pORI28 + 310-bp insert from lr1291 | This study |
| pKW05 | pORI28 + 152-bp insert from lr1351 | This study |
| pKW06 | pORI28 + 240-bp insert from lr1706 | This study |