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. 2008 Feb 1;74(6):1954–1958. doi: 10.1128/AEM.02294-07

FIG. 1.

FIG. 1.

Localization of the promoter activated after cellular TBT exposure in E. coli strain TBT3. (A) Map of the insertion region of the transposon in the chromosome of E. coli TBT3. H, HindIII site used for the cloning procedure. The double slash on the stpA arrow indicates the insertion site of the transposon in the stpA gene. The dotted line box delimits the region studied by deletion analysis, shown in panel B. (B) Localization of the promoter activated after cellular exposure to different TBT concentrations using deletion analysis of the upstream region of the luxAB insertion. The different regions of E. coli TBT3 tested for induction with TBT are represented by thick lines (numbers on each side indicate the base pair position from the transposon insertion). Increasing concentrations of TBT used (0, 0.1, 0.5, 1, 2, 5, and 10 μM) are indicated for each result by a rectangle with a gradient from white (0 μM) to black (10 μM). Experimental relative luminescence units/s (RLU/s) values represent the means of three independent experiments. Plasmid pBlux is a control plasmid devoid of an insert upstream of the luxCDABE reporter genes.