TABLE 1.
Oligonucleotides used for PCR amplifications
| Primer name | Restriction site | Nucleotide sequencea |
|---|---|---|
| Deletion analysis | ||
| TBT1F | HindIII | 5′-aggtaaagctt-TGCACCCAGAACGCGTGAAT-3′ |
| TBT1R | HindIII | 5′-acagcaagctt-TTACGAGTGGTATTTGACGATGTTGGC-3′ |
| TBT2F | NruI | 5′-gagtatcgcga-TGCACCCAGAACGCGTGAAT-3′ |
| TBT2R | EcoRI | 5′-agttgaattcctcctcct-GCACTGGCCTGTAATTGCGTGA-3′ |
| TBT3F | NruI | 5′-tattatcgcga-TCACGCAATTACAGGCCAGTGC-3′ |
| TBT4F | NruI | 5′-ccaagtcgcga-TAATGAACGCCCAACCGAACC-3′ |
| TBT5F | NruI | 5′-actcttcgcga-TGATGACGATTCTCCAGAAACCCA-3′ |
| TBT6F | NruI | 5′-agttttcgcga-GCGTAGTAATTTTAGCGGAGGCTG-3′ |
| TBT7F | NruI | 5′-aatgttcgcga-TCATTTACGCTGCTGAGGCTGG-3′ |
| TBT8R | EcoRI | 5′-agttgaattcctcctcct-CCAGCCTCAGCAGCGTAAATGA-3′ |
| Complementation of ygaVP deletion | ||
| TBT9R | EcoRI | 5′-tattgaattcctcctcct-CATGCGGCGAAATGGTTGTC-3′ |
| TBT10R | EcoRI | 5′-aaaaaagaattccctcctta-GTCCGGCGTCGCTTCTCAA-3′ |
a
Lowercase letters indicate linker sequences. Underlined letters indicate the restriction sites. Boldface letters indicate a Shine-Dalgarno sequence added, when necessary, for luxC translation.