TABLE 1.
qPCR primers and TaqMan probes designed for this study
| Type | Sequence (position) ofa:
|
Reference sequence | ||
|---|---|---|---|---|
| Primer
|
Probe | |||
| Reverse | Forward | |||
| Group A | TCAGGACCACCGGACTCAAC (146-127) | TAGCTGCAGAAAGAGGAACTGTAGAAG (50-76) | TAATTCCTGGCTATAACAAC (98-117) | AF059627.1 |
| Filamentous | GCAAATCCACCGCAAACAAC (275-256) | TGGCCGTGGTATTATTACTGCTATC (165-189) | AAGGAGCTTATACAGATCTA (206-225) | AY896367.1 |
| Group B | TCAGGACCACCAGATTCTACACACT (146-122) | TGCTGAAATGGGTTCTGTTGAA (54-75) | CGAAGACGTAATGCTC (87-102) | AY896454.1 |
| Group C | GGTATCCTTCAAGTAGTACTTCGTCTAGCT (112-83) | TCTACCCGTTTGATGCTACACACTAA (1-26) | AAACTACCATTCTTCACTTAGCAG (32-55) | AY896461.1 |
| Gamma A | AACAATGTAGATTTCCTGAGCCTTATTC 321-294 | TTATGATGTTCTAGGTGATGTG (240-266) | TTGCAATGCCTATTCG (275-290) | AY896371.1 |
| Gamma P | CATCGCGAAACCACCACATAC (282-262) | TTGTGCAGGTCGTGGTGTAATC (159-180) | CCTATGACGAAGACCTAGAC (212-231) | AY896428.1 |
| Cluster III | GCAGACCACGTCACCCAGTAC (267-247) | ACCTCGATCAACATGCTCGAA (175-195) | CCTGGACTACGCGTTC (225-240) | AY896461.1 |
a
All positions and sequences relate to the 324-base nifH segment length (5′→3′).