FIG. 5.

Deletion identified in the LAC12-LAC4 intergenic region of the plasmid isolated from the evolved strain (T1-E). The 1,593-bp deletion (open box) was mapped between positions −516 and −2108, +1 referring to the adenosine in the LAC4 initiation codon. The functional UAS elements (U) present in the LAC promoter (13) are represented by vertical black bars and correspond to the 17-bp consensus sequence 5′-CGG(N5)A/T(N5)CCG-3′ (in the figure, the central position of the consensus sequence at each UAS is indicated in parentheses). The gray vertical bar represents an additional putative UAS (pU) found in T1 and T1-E (see the text for details).