TABLE 1.
Oligonucleotides used for quantitative RT-PCR
| Oligonucleotide | Sequence | Amplicon size (bp) | Efficiencya |
|---|---|---|---|
| ACT1_QPCR_F1bis | ATTATATGTTTAGAGGTTGCTGCTTTGG | 285 | 1.98 |
| ACT1_QPCR_R1bis | CAATTCGTTGTAGAAGGTATGATGCC | ||
| Bla_QPCR_F13 | CATTTCCGTGTCGCCCTTATTCCC | 147 | 2.11 |
| Bla_QPCR_R159 | CTTACCGCTGTTGAGATCCAGTTCG | ||
| LAC12_QPCR_F742 | GGTCTTGTGTGTATATTTGGTTGGTTAATCCC | 246 | 1.97 |
| LAC12_QPCR_R987 | TGCTCTGTACCTATCCGATCTCGTTCTG | ||
| LAC4_QPCR_F1733 | GTGGCTTTATCTGGGAATGGGCAAATC | 278 | 2.03 |
| LAC4_QPCR_R2010 | CAATAAGTGGTCTGTCGTAATGAAGTCGTG |
a
Efficiency (E) was determined using the formula E = 10(−1/slope), with “slope” being the slope of the standard curve that was obtained from serial dilution of sample cDNA.