TABLE 3.
Relative expression of bla, LAC4, and LAC12 genes during growth of T1 and T1-E in lactose and in glucose (after normalization with ACT1)a
| Gene | % Expression during growth in lactose of:
|
% Expression during growth in glucose of:
|
||
|---|---|---|---|---|
| T1 | T1-E | T1 | T1-E | |
| bla | 100 ± 1 | 8 ± 0 | 50 ± 5 | 6 ± 1 |
| LAC4 | 100 ± 4 | 225 ± 6 | 69 ± 2 | 7 ± 2 |
| LAC12 | 100 ± 3 | 45 ± 5 | 66 ± 5 | 5 ± 2 |
a
Expression levels were determined by quantitative real-time RT-PCR, as described in Materials and Methods. Expression levels of bla, LAC4, and LAC12 were normalized using ACT1 as the reference gene. T1 cells grown in lactose were used as the calibrator sample (the expression was set at 100%). Results shown are means ± ranges of results from duplicate biological cultivations. Each sample (one sample from each biological duplicate) was analyzed in duplicate, and the coefficient of variation (after normalization) between the results for these technical duplicates was <30%.