TABLE 4.

Relative expression of LAC4 and LAC12 genes during growth of T1 and T1-E in lactose and in glucose (after normalization with bla)^a

Gene	% Expression during growth in lactose of:		% Expression during growth in glucose of:
	T1	T1-E	T1	T1-E
LAC4	100 ± 3	2,677 ± 211	138 ± 17	103 ± 12
LAC12	100 ± 4	531 ± 30	133 ± 21	76 ± 19

^aExpression levels were determined by quantitative real-time RT-PCR, as described in Materials and Methods. Expression levels of LAC4 and LAC12 were normalized using bla as the reference gene. T1 cells grown in lactose were used as the calibrator sample (the expression was set at 100%). Results shown are means ± ranges of results from duplicate biological cultivations. Each sample (one sample from each biological duplicate) was analyzed in duplicate, and the coefficient of variation (after normalization) between the results for these technical duplicates was <30%.