TABLE 1.

Probes used for multiplex genotyping of C. trachomatis VS1-VS2 PCR products

Probe	GenBank accession no.	Probe sequence (5′-3′)	Probe signal (MFI)a		Minimum ratiob
			Negative range	Positive range
Bac	AY950630	GAGAACCAGACTAAAGTTTCAA	0-32	136	3.8
Dd	X62918	AAAAAACGGTCAAAGCGGAGTC	0-45	225-851	5.0
Ed	X52557	ACAGATACTGCCTTCTCTTGG	0-31	225-977	7.3
F	X52080	ATCTGCAGCAGGTTTCGTGG	0-25	105-311	4.2
G	AF063199	CAGGCTGCGTGGCGTTTT	0-31	602-791	19.4
Hd	X16007	ACAAAATCTTCTGATTTTAATACAGC	0-24	163-426	6.8
J	AF063202	TCTTTTTCCTAACACCGCTTTGAA	0-28	68-102	2.4
Kd	AF063204	AACACTGCTTTGGATCGAGCTGTG	0-41	171-271	4.2

^aThe negative range is the range of the median fluorescence intensity (MFI) for all negative strains after the subtraction of the background for the given primer. The positive range is the range of the MFI for all positive strains after the subtraction of the background for the given primer.

^bThe minimum ratio is the lowest recorded positive value divided by the highest recorded negative value. A minimum ratio of >2 is used as the threshold for defining positive events.

^cThe C. trachomatis type-specific probe Ba has previously been published (13).

^dThese C. trachomatis type-specific probes, D, E, H, and K, have previously been published (11).