TABLE 2.
Detection (minimum ratios) of different genotypes mixed 10:1 in individual specimens
| Plasmid DNA genotype at onefold concn | Minimum ratio(s) of indicated plasmid DNA genotype at 10-fold concna
|
Unique (minimum ratio)b | |||||||
|---|---|---|---|---|---|---|---|---|---|
| D | E | F | G | J | K | B | H | ||
| D | 10.3 | 29.7/7.7 | 13.5/27.3 | 17.3/32.2 | 10.6/16.6 | 9.4/16.5 | 4.0/19.0 | 6.0/16.9 | 16.7 |
| E | 6.4/46.1 | 52.7 | 7.6/46.9 | 20.7/28.5 | 18.7/34.6 | 14.3/45.5 | 12.0/29.1 | 13.3/52.4 | 57.6 |
| F | 15.4/3.5 | 39.4/6.6 | 6.4 | 9.1/1.8c | 11.4/5.0 | 17.5/5.3d | 13.3/7.8 | 14.3/3.0 | 3.4 |
| G | 17.5/4.8 | 38.2/9.2 | 5.6/5.3 | 25.4 | 10.3/12.1 | 12.0/13.2 | 19.2/21.4 | 16.0/21.1 | 19.3 |
| J | 12.8/3.6 | 43.6/13.0 | 8.0/9.6 | 21.7/9.7 | 17.7 | 21.2/3.5d | 22.4/14.6 | 16.1/10.4d | 17.9 |
| K | 30.6/5.6 | 43.0/5.8 | 3.9/5.2 | 14.2/9.1 | 8.6/2.8 | 17.8 | 9.6/19.5 | 17.0/3.6 | 8.5 |
| B | 33.2/10.4 | 46.7/9.1 | 8.0/19.9 | 27.5/8.5 | 9.6/8.9 | 18.3/12.8 | 23.5 | 21.0/31.4 | 16.1 |
| H | 15.5/18.2 | 47.4/31.0 | 3.3/19.1 | 29.9/5.9 | 2.0/8.0 | 18.3/11.6 | 22.5/27.8 | 15.6 | 13.7 |
Plasmid DNA of the indicated genotypes was mixed at a ratio of 10:1 for one PCR. The boldface values are the minimum ratios of the 10-fold-concentration plasmid DNA of the genotypes indicated in boldface at the top of the table. The lightface values are the minimum ratios of the onefold-concentration plasmid DNA of the genotypes indicated in lightface in the left-hand column.
Unique, only a onefold concentration of plasmid DNA of one single genotype was used in one PCR.
A twofold concentration of plasmid DNA of genotype G was mixed with a onefold concentration of plasmid DNA of genotype F.
Onefold concentrations of plasmid DNA of genotypes K and H were mixed with onefold concentrations of plasmid DNA of genotypes F and J, respectively.