TABLE 2.
MLST polymorphisms in ITS1 and β-tub regions of DNA from P. jirovecii isolated from patients 1 to 20
| Patient no. | Nucleotide(s) in ITS1 region at position(s) (bp):
|
ITS1 allelea | Base in β-tub region at position (bp):
|
β-tub allelea | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2 | 8-10 | 11 | 17 | 22 | 46-47 | 54-62 | 71-72 | 111-113 | 24 | 282 | |||
| 1 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 2b | |||||||||||||
| 3 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 4 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 5 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 6 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 7 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 8 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 9b | |||||||||||||
| 10 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 11 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 12 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 13e | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | |||
| 14 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 15 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 16 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 17 | T | 2× T | A | T | T | TC | 10× T | GAGG | TTA | B | A | G | 1 |
| 18 | T | 2× T | A | T | T | TC | 9× T | GAGG | TTA | B1 | A | G | 1 |
| 19 | T | 3× T | A | T | C/Tc | TC | 10× T | GAGG | TTA | D1d | A | G | 1 |
| 20 | T | 3× T | A | T | T | TC | 10× T | GAGG | TTA | Dd | A | G | 1 |
a
Alleles were determined as described by Hauser et al. (8).
b
BAL samples from patients 2 and 9 were not available for MLST typing.
c
Sequence not clearly identifiable; possibly more than two types.
d
New allele, identified in this study.
e
There was no PCR product for β-tub DNA from P. jirovecii isolated from patient 13.