TABLE 1.
Primers used for sequencing the gap gene
| Primer | Sequence (5′→3′) | Positiona |
|---|---|---|
| Gap1-forb | ATGGTTTTGGTAGAATTGGTCGTTTA | 22-47 |
| Gap3-revc | G(ACT)TTT(AGCT)A(CT)TTCTT(AGT) (AT)CC(AG)TT(ACT)AC(AGT)C | 220-196 |
| Gap4-forc | GA(CT)GT(AGCT)GT(AGCT)(CT)T(AT)GAATGTAC(AT)GG | 270-292 |
| Gap5-revc | GTT(AT)GT(AT)GTACA(AGT)GA(ACT)GCACC(AT)G | 462-440 |
| Gap6-forc | GAAGG(ACT)(CT)T(ACT)ATGAC(AGT)AC(AT)AT(CT)CA(CT)G | 511-535 |
| Gap7-revc | GAACC(AT)GT(AT)GC(AT)AC(AT)GG (ACT)ACACGTTG | 723-698 |
| Gap8-forc | GAA(ACT)CATT(CT)GGTTACA(AC) (ACT)GA(AT)GA(CT)G | 809-834 |
| Gap2-revb | GACATTTCGTTATCATACCAAGCTG | 956-932 |
a
Position relative to the S. aureus gap sequence.
b
Primer sequences from Yugueros et al. (36).
c
Designed primer sequences from this study.