TABLE 1.
Molecular and genotypic features of Staphylococcus aureus and CoNS control strainsa
| Strain type and name | PFGE type (pattern)b | spa type | Molecular type | Genotype (presence of indicated gene or marker)c
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Staphylococcus sp. specific (16S rRNA) | S. aureus specific (nuc) | Methicillin resistant (mecA) | PVL specific (PVL) | Phage (MW1409) | USA400 (MW0756) | USA300 (arcA) | ||||
| PVL+ USA300 | ||||||||||
| CMRSA10 | USA300-0114 (A) | t008 | ST8-MRSA-IVa | + | + | + | + | − | + | |
| PMRSA-13 | USA300-0114 (A) | t008 | STnew-MRSA-Ivad | + | + | + | + | + | − | + |
| PMRSA-16 | USA300-0114 (A) | t008 | ST8-MRSA-IVa | + | + | + | + | + | − | + |
| PMRSA-46 | USA300-0114 (A) | t008 | ST8-MRSA-IVa | + | + | + | + | + | − | + |
| PMRSA-12 | USA300 (B) | t008 | ST8-MRSA-IVa | + | + | + | + | + | − | + |
| C21744 | USA300 (C) | t008 | ST8-MRSA-IVa | + | + | + | + | + | − | + |
| PVL− USA400 | ||||||||||
| C2901 | USA400 (B) | t128 | ST1-MRSA-IVa | + | + | + | − | − | + | − |
| C2140 | USA400 (A) | t128 | ST1-MRSA-IVa | + | + | + | − | − | + | − |
| PVL+ USA400 | ||||||||||
| CMRSA7 | USA400 (A) | t128 | ST1-MRSA-IVa | + | + | + | + | + | + | − |
| PMRSA-18 | USA400 (A) | t128 | ST1-MRSA-IVa | + | + | + | + | + | + | − |
| PMRSA-50 | USA400 (A) | t128 | ST1-MRSA-IVa | + | + | + | + | + | + | − |
| C10687 | USA400 (A) | t128 | ST1-MRSA-IVa | + | + | + | + | + | + | − |
| C6413 | USA400 (C) | t128 | ST1-MRSA-IVa | + | + | + | + | + | + | − |
| PVL− MRSA | ||||||||||
| CMRSA1 | USA600 | t004 | ST45-MRSA-II | + | + | + | − | − | − | − |
| CMRSA2 | Like USA100/800 | t002 | ST5-MRSA-II | + | + | + | − | − | − | − |
| C4000 | Like USA100/800 | t004 | ST225-MRSA-II | + | + | + | − | − | − | − |
| CMRSA3 | Like EMRSA1/4/11 | t037 | ST241-MRSA-III | + | + | + | − | − | − | − |
| CMRSA6 | Like EMRSA1/4/11 | t037 | ST239-MRSA-III | + | + | + | − | − | − | − |
| C1777 | Like EMRSA1/4/11 | t037 | ST239-MRSA-III | + | + | + | − | − | − | − |
| CMRSA4 | USA200/EMRSA16 | t018 | ST36-MRSA-II | + | + | + | − | − | − | − |
| C23374 | USA200/EMRSA 16 | t018 | ST36-MRSA-II | + | + | + | − | − | − | − |
| CMRSA5 | USA500 | t064 | ST8-MRSA-IVd | + | + | + | − | + | − | − |
| CMRSA8 | EMRSA 15 | t022 | ST22-MRSA-IV | + | + | + | − | − | − | − |
| CMRSA9 | t008 | ST8-MRSA | + | + | + | − | − | − | − | |
| C74 | t1154 | ST5-MRSA-IVb | + | + | + | − | − | − | − | |
| PVL+ MRSA | ||||||||||
| H435 | t311 | ST5-MRSA-II | + | + | + | + | − | − | − | |
| PMRSA-34 | t437 | ST59-MRSA-III | + | + | + | + | − | − | − | |
| MR37 | Like USA400 | t175 | ST1-MRSA-IVa | + | + | + | + | − | − | − |
| MR138 | t044 | ST80-MRSA-IVa | + | + | + | + | − | − | − | |
| H434 | Like USA1100 | t019 | ST30-MRSA IVc | + | + | + | + | − | − | − |
| PMRSA-29 | USA1100 | t019 | ST30-MRSA-IVc | + | + | + | + | − | − | − |
| C1538 | USA1100 | t019 | ST30-MRSA-IVc | + | + | + | + | − | − | − |
| PVL+ MSSA | ||||||||||
| MS02-W10 | USA800 | t015 | ST5-MSSA | + | + | − | + | − | − | − |
| SA5 | Unnamede | ST121-MSSA | + | + | − | + | − | − | − | |
| SA112 | t645 | ST121-MSSA | + | + | − | + | − | − | − | |
| SA125 | t436 | ST25-MSSA | + | + | − | + | − | − | − | |
| SA134 | t005 | ST22-MSSA | + | + | − | + | − | − | − | |
| SA28 | t437 | ST59-MSSA | + | + | − | + | − | − | − | |
| MS03-B1 | t437 | ST59-MSSA | + | + | − | + | − | − | − | |
| SA3 | Unnamede | ST30-MSSA | + | + | − | + | − | − | − | |
| H49 | t021 | ST30-MSSA | + | + | − | + | − | − | − | |
| SAF516 | t483 | ST30-MSSA | + | + | − | + | + | − | − | |
| PVL− MS-CoNS | ||||||||||
| S. epidermidis (ATCC12228) | ACME+ | + | − | − | − | − | − | + | ||
| PVL− MS-CoNS | ||||||||||
| S. epidermidis (CNS99-PF5) | ACME− | + | − | − | − | − | − | − | ||
| PVL− MS-CoNS | ||||||||||
| S. epidermidis (CNS99-PF7) | ACME+ | + | − | − | − | − | − | + | ||
| PVL− MR-CoNS | ||||||||||
| S. epidermidis (GISE 12333) | ACME+ | + | − | + | − | − | − | + | ||
| PVL− MR-CoNS | ||||||||||
| S. epidermidis (CNS99-PF6) | ACME− | + | − | + | − | − | − | − | ||
| PVL− MR-CoNS | ||||||||||
| S. epidermidis (CNS99-PF8) | ACME+ | + | − | + | − | − | − | + | ||
PFGE, PVL gene typing, SCCmec typing, spa typing, and MLST were used for strain characterization. The identification of MRSA isolates matching the USA300 and USA400 CA-MRSA strains was based on the similarity of PFGE patterns to those of the USA300 and USA400 control strains and the presence of PVL, SCCmec type IVa, spa type t008, and MLST type ST8 (for USA300) and of PVL, SCCmec type IVa, spa type t128, and MLST type ST1 (for USA400).
PFGE patterns A (indistinguishable from the USA300 control strain [CMRSA10]), B (an additional band around 150 kb in size), and C (the band at around 250 bp shifting to around 310 kb) for strain USA300 and patterns A, B, and C for strain SA400 (see reference 13 for details of the patterns) are indicated in brackets.
+, present; −, absent.
New ST (MLST) profile: PMRSA-13 (3-3-1-4-4-4-3).
The spa type profiles are unnamed for SA5 (I2H2M) and SA3 (I2H2M).