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. 2008 Jan 22;28(6):1988–1998. doi: 10.1128/MCB.01442-07

FIG. 5.

FIG. 5.

SnoN is essential for TGF-β-mediated AFP repression. (A) SnoN recruitment to SBE/p53RE is independent of p53. Quantified ChIP analysis for SnoN protein bound to SBE/p53RE was performed with a SnoN-specific antibody as described in the legend to Fig. 3. (A and B) Stable depletion of SnoN compromises AFP repression in response to TGF-β. (B) Analysis of SnoN protein levels. Control and SnoN KD Hepa 1-6 cells were treated with vehicle (V) or TGF-β and harvested for nuclear extract preparation. Standard Western blot analysis was performed to determine the levels of SnoN protein depletion. Actin levels were used to control for loading errors. (C) Quantitative RT-PCR analysis of SnoN RNA was performed as described in the legend to Fig. S1 in the supplemental material to ensure maintenance of SnoN KD under the experimental conditions shown in panel D. (D) Quantitative RT-PCR analysis of AFP RNA was performed as described in the legend to Fig. 2A for the indicated cell types. Black and gray bars indicate vehicle- and TGF-β-treated cells, respectively.