FIG. 4.

Overexpression of telomerase proteins in the reg234Δ asc mutant. High-copy-number plasmids harboring telomerase proteins were introduced into the reg234Δ asc mutant, as described in the legend of Fig. 3, grown at 30°C (A and B) or 25°C (C) for six passages, and analyzed by Southern blot hybridization, as described in the legend of Fig. 1. In panel C, the entire gel, including the 12 K. lactis telomeres, is shown. Asterisks indicate missing telomeric fragments, presumably due to subtelomeric recombination. Only BclI-specific hybridization is shown for the 25°C experiment.