FIG. 5.

Overexpression of telomerase proteins in a reg234Δ asc rad52Δ double mutant. (A) Deletion of the RAD52 gene was combined with the reg234Δ asc, WT, and ter1Δ strains and grown at 30°C (shown is the third passage). (B) High-copy-number plasmids harboring telomerase proteins were introduced into the reg234Δ asc rad52Δ double mutant and grown at 30°C (shown is the sixth passage). (C) Genomic DNA was prepared from these strains at their sixth passage and analyzed by Southern blot hybridization, as described in the legend of Fig. 1. Only hybridization to the WT telomeric probe is shown.