FIG. 6.

RE-IIBP expression and histone methylation levels are increased in leukemia patient samples. (A) Nuclear extracts from leukemic cell lines K562 and HL-60 amplified with primers for the 5′ UTR RE-IIBP region were immunoblotted with anti-dimethyl H3-K27 antibodies. (B) Semiquantitative RT-PCR analysis was performed with primers for the 5′ UTR RE-IIBP region. Human glyceraldehyde-3-phosphate dehydrogenase (hGAPDH) was used as an RNA loading control. (C) Histones extracted from blood cells of healthy individuals and from leukemia patients were separated by 18% SDS-PAGE and immunoblotted against anti-dimethyl H3-K27 and anti-pan methyl lysine antibodies. The loading of equal amounts of sample was confirmed by Western blotting with antibodies against histone H3.