Growth at 30°C of BTN1+ and btn1-Δ strains on YPG containing 1.33 mg/ml of ANP, and YPD containing 2 mg/ml of ANP. (A) B-7553 (BTN1+ CYC1+), YPG, 4 days. (B) B-10195 (btn1-Δ CYC1+), YPG, 4 days. (C) B-7553 (BTN1+ CYC1+), YPG + ANP, 7 days. (D) B-10195 (btn1-Δ CYC1+), YPG + ANP, 7 days. (E) B-6748 (BTN1+ cyc1-Δ), YPD, 4 days. (F) B-10196 (btn1-Δ cyc1-Δ), YPD, 4 days. (G) B-6748 (BTN1+ cyc1-Δ), YPD + ANP, 7 days. (H) B-10196 (btn1-Δ cyc1-Δ), YPD + ANP, 7 days. Serial dilutions (1/10) of the strains were spotted on the various media.