TABLE 2.
Identification of some expressed MLV provirusesa
cDNA group and provirus no.b | Provirus type | Frequency (no. of hits) |
---|---|---|
cDNAs from normal liver | ||
1 | Intact, in Ctse | 2 |
22 | Intact, in Galnt11 | 4 |
32 | Intact | 2 |
38 | pro-pol deletedc | 1 |
68 | pro-pol and env deletedc | 7 |
Not assignedd | 6 | |
cDNAs from macroH2A1 knockout liver | ||
32 | Intact | 2 |
62 | Intact | 1 |
38 | pro-pol deletedc | 1 |
29 | env deleted | 2 |
39 | env deleted | 5 |
70 | env deleted | 4 |
68 | pro-pol and env deletedc | 1 |
Not assignedd | 7 |
Cloned MLV cDNAs from the pro-pol region were sequenced and compared to the public genomic database for C57BL/6 mice (build 37.1). See Table S3 in the supplemental material for primer sequences.
Proviruses were numbered according to their occurrence in the public genomic database for C57BL/6 mice (see Table S2 in the supplemental material).
These pro-pol deletions ended before the start of the pro-pol segment that we amplified for this analysis.
Sequences were not assigned to a provirus when they matched multiple proviruses or had more than one mismatch to their best hit.